Strain Data Sheet

RBRC10060

Strain Information

Image
BRC No.RBRC10060
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-F8<tm1.1(EGFP)Tsuka>
Former Common nameF8 EGFP knockin flox/flox mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Tsukasa Ohmori, Jichi Medical University. Bruce 4 ES cell line was used. C57BL/6 congenic line.
Strain descriptionF8 (coagulation factor VIII) floxed mice with an inducible EGFP expression. Exon 16 of F8 is replaced with the following DNA fragment; loxP-F8 cDNA (exon 16–28)-SV40 pA-loxP-splice acceptor-P2A-EGFP-SV40 pA. In the presence of Cre, cDNA (exon 16–28) can be deleted and EGFP starts to express. Even in the absence of Cre, there is some EGFP expression due to splicing. RBRC10061 is a strain in which Cre deleted DNA fragment between loxP sites.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)XEGFP
F8
MGI:88383		coagulation factor VIIIXF8<tm1.1(EGFP)Tsuka> targeted mutation 1.1, Tsukasa Ohmori
  • hemophilia A(DisGeNET, MedGEN)
    • Frt yeast FRT (flippase recombination target) siteXFrt
    P2A porcine teschovirus-1 2A peptideXP2A
    loxP phage P1 loxPXloxP
    loxP phage P1 loxPXloxP
    SV40 polyAX

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, SA, mouse F8 cDNA, Simian Virus (SV40) PolyA, Saccharomyces cerevisiae Frt site, P2A (porcine teschovirus-1 2A peptide), jellyfish EGFP cDNA, mouse F8 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorTsukasa Ohmori (Jichi Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data