Strain Information | |
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Image | |
BRC No. | RBRC10061 |
Type | Targeted Mutation Congenic![]() |
Species | Mus musculus |
Strain name | B6.Cg-F8<tm1.2(EGFP)Tsuka> |
Former Common name | F8 EGFP knockin mice |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Tsukasa Ohmori, Jichi Medical University. Bruce 4 ES cell line was used. C57BL/6 congenic line. |
Strain description | EGFP knock-in mice at the F8 (coagulation factor VIII) gene locus. EGFP was placed at downstream of Exon 16. F8 activity is less than 1% in homozygous mice. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | X | EGFP | ||||
F8 MGI:88383 | coagulation factor VIII | X | F8<tm1.2(EGFP)Tsuka> | targeted mutation 1.2, Tsukasa Ohmori | |||
P2A | porcine teschovirus-1 2A peptide | X | P2A | ||||
loxP | phage P1 loxP | X | loxP | ||||
SV40 polyA | X |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 loxP site, SA, mouse F8 cDNA, P2A (porcine teschovirus-1 2A peptide), jellyfish EGFP cDNA, Simian Virus (SV40) PolyA, mouse F8 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Tsukasa Ohmori (Jichi Medical University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |