Strain Data Sheet

RBRC10063

Strain Information

Image
BRC No.RBRC10063
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Foxp3<tm1(CD2/CD52)Shori>
Former Common nameB6.Foxp3hCD2
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shohei Hori, RIKEN Center for Integrative Medical Sciences. C57BL/6J-derived ES cells were used. The neo gene was removed by crossing to CAG-Cre mice. C57BL/6J genetic background.
Strain descriptionFoxp3 (forkhead box P3) reporter mice. A DNA fragment containing IRES and cDNA for human CD2-human CD52 fusion protein was knocked in at 3’ UTR (between stop codon and endogenous polyA) of the Foxp3 gene.
Colony maintenance
References
Plasticity of Foxp3(+) T cells reflects promiscuous Foxp3 expression in conventional T cells but not reprogramming of regulatory T cells.
Miyao T, Floess S, Setoguchi R, Luche H, Fehling H J, Waldmann H, Huehn J, Hori S
Immunity, 36(2):262-75 (2012). 22326580

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
CD2 CD2 (human)XCD2
CD52 CD52 antigenXCD52
Foxp3
MGI:1891436		forkhead box P3XFoxp3<tm1(CD2/CD52)Shori>
MGI:4950682		targeted mutation 1, Shohei Hori
  • immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome(MedGEN)
    • Ires internal ribosomal entry site (EMCV)X
    loxP phage P1 loxPXloxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased regulatory T cell number(MP:0004974)

  • increased susceptibility to type IV hypersensitivity reaction(MP:0005617)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human CD2 cDNA, human CD52 cDNA, Enterobacteria phage P1 loxP site, mouse Foxp3 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 36(2):262-75 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorShohei Hori (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Hattori K, Tanaka S, Hashiba D, Tamura J, Etori K, Kageyama T, Ito T, Meguro K, Iwata A, Suto A, Suzuki K, Nakamura J, Ohtori S, Ziegler SF, Nakajima H.
    Synovial regulatory T cells expressing ST2 deteriorate joint inflammation through the suppression of immunoregulatory eosinophils.
    J Autoimmun 149 103333(2024) 39509740