Strain Data Sheet

RBRC10064

Strain Information

Image
BRC No.RBRC10064
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Foxp3<tm2.1(EGFP/Cre)Shori>
Former Common nameB6.Foxp3eGFPCre
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shohei Hori, RIKEN Center for Integrative Medical Sciences. M1 ES cells (C57BL/6Jx129/Sv F1) were used. The neo gene was removed by crossing to CAG-Cre mice, and then the mice were backcrossed to C57BL/6J over 12 generations. C57BL/6 congenic line.
Strain descriptionKnockin mice expressing eGFP-Cre under the control of the Foxp3 (forkhead box P3) gene locus. A DNA fragment containing IRES and eGFP-Cre was knocked in at 3’ UTR (between stop codon and endogenous polyA) of the Foxp3 gene.
Colony maintenance
References
Plasticity of Foxp3(+) T cells reflects promiscuous Foxp3 expression in conventional T cells but not reprogramming of regulatory T cells.
Miyao T, Floess S, Setoguchi R, Luche H, Fehling H J, Waldmann H, Huehn J, Hori S
Immunity, 36(2):262-75 (2012). 22326580

Health Report

Examination Date / Room / Rack2024/12/09Room:3-6Rack:DSentinel mouse program
2024/09/09Room:3-6Rack:DSentinel mouse program
2024/06/10Room:3-6Rack:DSentinel mouse program
2024/03/11Room:3-6Rack:DSentinel mouse program
2023/12/11Room:3-6Rack:DSentinel mouse program
2023/09/22Room:3-6Rack:DAdditional tests
2023/09/11Room:3-6Rack:DSentinel mouse program
2023/06/13Room:3-6Rack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Foxp3
MGI:1891436		forkhead box P3XFoxp3<tm2.1(EGFP/Cre)Shori>
MGI:5609975		targeted mutation 2.1, Shohei Hori
  • immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome(MedGEN)
    • Frt yeast FRT (flippase recombination target) siteXFrt
    GFP Green Fluorescent Protein (Aequorea victoria)X
    Ires internal ribosomal entry site (EMCV)X
    cre Phage P1 Cre recombinaseXcre

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased regulatory T cell number(MP:0004974)
    • Detailed phenotype data

    Ordering Information

    Donor DNAECMV (Encephalomyocarditis virus) IRES, Aequorea victoria GFP (eGFP) cDNA, Enterobacteria phage P1 Cre, Saccharomyces cerevisiae Frt site, mouse Foxp3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 36(2):262-75 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorShohei Hori (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data