Strain Data Sheet

RBRC10064

Strain Information

Image
BRC No.RBRC10064
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Foxp3<tm2.1(EGFP/Cre)Shori>
Former Common nameB6.Foxp3eGFPCre
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shohei Hori, RIKEN Center for Integrative Medical Sciences. M1 ES cells (C57BL/6Jx129/Sv F1) were used. The neo gene was removed by crossing to CAG-Cre mice, and then the mice were backcrossed to C57BL/6J over 12 generations. C57BL/6 congenic line.
Strain descriptionKnockin mice expressing eGFP-Cre under the control of the Foxp3 (forkhead box P3) gene locus. A DNA fragment containing IRES and eGFP-Cre was knocked in at 3’ UTR (between stop codon and endogenous polyA) of the Foxp3 gene.
Colony maintenance
References
Plasticity of Foxp3(+) T cells reflects promiscuous Foxp3 expression in conventional T cells but not reprogramming of regulatory T cells.
Miyao T, Floess S, Setoguchi R, Luche H, Fehling H J, Waldmann H, Huehn J, Hori S
Immunity, 36(2):262-75 (2012). 22326580

Health Report

Examination Date / Room / Rack2025/06/09Room:3-6Rack:DSentinel mouse program
2025/03/10Room:3-6Rack:DSentinel mouse program
2024/12/09Room:3-6Rack:DSentinel mouse program
2024/09/09Room:3-6Rack:DSentinel mouse program
2024/06/10Room:3-6Rack:DSentinel mouse program
2024/03/11Room:3-6Rack:DSentinel mouse program
2023/12/11Room:3-6Rack:DSentinel mouse program
2023/09/22Room:3-6Rack:DAdditional tests
2023/09/11Room:3-6Rack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Foxp3
MGI:1891436
forkhead box P3XFoxp3<tm2.1(EGFP/Cre)Shori>
MGI:5609975
targeted mutation 2.1, Shohei Hori
  • immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome(DisGeNET, MedGEN)
  • Frt yeast FRT (flippase recombination target) siteXFrt
    GFP Green Fluorescent Protein (Aequorea victoria)X
    Ires internal ribosomal entry site (EMCV)X
    cre Phage P1 Cre recombinaseXcre

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • decreased regulatory T cell number (MP:0004974)
  • Detailed phenotype data

    Ordering Information

    Donor DNAECMV (Encephalomyocarditis virus) IRES, Aequorea victoria GFP (eGFP) cDNA, Enterobacteria phage P1 Cre, Saccharomyces cerevisiae Frt site, mouse Foxp3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 36(2):262-75 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorShohei Hori (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data