Strain Data Sheet

RBRC10074

Strain Information

Image
BRC No.RBRC10074
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ebag9<tm1.1Sinos>
Former Common nameEBAG9 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Satoshi Inoue, Saitama Medical University (2014). E14 ES cells (129P2/OlaHsd) were used. Mice were backcrossed to C57BL/6N over 5 generations.
Strain descriptionKnockout mouse of Ebag9 (estrogen receptor-binding fragment-associated gene 9). Exon 2 is deleted.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2023/10/10Room:3-BRack:ASentinel mouse program
2023/07/10Room:3-BRack:ASentinel mouse program
2023/04/10Room:3-BRack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ebag9
MGI:1859920		estrogen receptor-binding fragment-associated gene 915Ebag9<tm1.1Sinos> targeted mutation 1.1, Satoshi Inoue
loxP phage P1 loxP15loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased metastatic potential(MP:0001273)

  • decreased tumor growth/size(MP:0003447)

  • increased CD4-positive, alpha-beta T cell number(MP:0008074)

  • increased CD8-positive, alpha-beta T cell number(MP:0008078)

  • increased NK cell degranulation(MP:0008792)
    • more 1 phenotypes
  • increased natural killer cell mediated cytotoxicity(MP:0003080)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Ebag9 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use the BIOLOGICAL RESOURSE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorSatoshi Inoue (Saitama Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data