Strain Data Sheet

RBRC10080

Strain Information

Image
BRC No.RBRC10080
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6J.Cg-Trp53<tm1Sia>
Former Common namep53KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6J and KSN-nu by Yoko Hirabayashi, National Institute of Health Sciences, C57BL/6N, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain descriptionTrp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. C57BL/6J congenic line. Congenic strains backcrossed to other inbred strains are also available as follows: C57BL/6N background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenance
References
Enhanced proliferative potential in culture of cells from p53-deficient mice.
Tsukada T, Tomooka Y, Takai S, Ueda Y, Nishikawa S, Yagi T, Tokunaga T, Takeda N, Suda Y, Abe S, et a l
Oncogene, 8, 3313-3322 (1993). 7504233

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trp53
MGI:98834		transformation related protein 5311Trp53<tm1Sia>
MGI:1926340		targeted mutation 1, Shinichi Aizawa
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
    • more 14 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)
    • neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell proliferation(MP:0000350)

  • decreased cellular sensitivity to ultraviolet irradiation(MP:0008411)

  • increased T cell derived lymphoma incidence(MP:0002024)

  • increased sensitivity to induced cell death(MP:0008943)

  • increased tumor incidence(MP:0002020)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHSV Tk promoter, Escherichia coli neomycin resistance gene (neo), HSV TK polyA, mouse Trp53 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 8, 3313-3322 (1993). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT is requested to mention the RIKEN Accession Number CDB 0001K (refer https://large.riken.jp/distribution/mutant-list.html) and to notice to RIKEN CDB LARGE (Laboratories of Animal Resource development and Genetic Engineering, mutant.bdr@riken.jp).
    DepositorYoko Hirabayashi (National Institute of Health Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data