Strain Data Sheet

RBRC10082

Strain Information

Image
BRC No.RBRC10082
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Kcnj11<tm2.1Sse>
Former Common nameKir6.2-floxed delta neo mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain descriptionKcnj11 (potassium inwardly rectifying channel, subfamily J, member 11, Kir6.2) floxed mice. The neo gene was removed by B6-Tg(CAG-FLPe)36.
Colony maintenance
References
Gs/Gq signaling switch in β cells defines incretin effectiveness in diabetes.
Okechi S Oduori, Naoya Murao, Kenju Shimomura, Harumi Takahashi, Quan Zhang, Haiqiang Dou, Shihomi Sakai, Kohtaro Minami, Belen Chanclon, Claudia Guida, Lakshmi Kothegala, Johan Tolö, Yuko Maejima, Norihide Yokoi, Yasuhiro Minami, Takashi Miki, Patrik Rorsman, Susumu Seino
J. Clin. Invest., 130(12):6639-6655 (2020). 33196462

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site7Frt
Kcnj11
MGI:107501		potassium inwardly rectifying channel, subfamily J, member 117Kcnj11<tm2.1Sse> targeted mutation 2.1, Susumu Seino
  • diabetes mellitus, permanent neonatal 2(MedGEN)

  • diabetes mellitus, transient neonatal, 3(MedGEN)

  • hyperinsulinemic hypoglycemia, familial, 2(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young type 13(MedGEN)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 2 diabetes mellitus(DisGeNET, MedGEN)
    • loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Yeast FRT (flippase recombination target) site, mouse Kcnj11 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorSusumu Seino (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data