Strain Data Sheet

RBRC10083

Strain Information

Image
BRC No.RBRC10083
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Map3k5<tm3.1Hijo>
Former Common nameAsk1 flox/flox, Map3k5 flox/flox
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. ENKA (C57BL/6) ES cells were used. C57BL/6 genetic background.
Strain descriptionFloxed Map3k5 (mitogen-activated protein kinase kinase kinase 5、ASK1) mice. Exon 15 was flanked by loxP sites.
Colony maintenance
References
ASK1 signalling regulates brown and beige adipocyte function.
Hattori K, Naguro I, Okabe K, Funatsu T, Furutani S, Takeda K, Ichijo H
Nat. Commun., 7:11158 (2016). 27045525

ASK1 facilitates tumor metastasis through phosphorylation of an ADP receptor P2Y12 in platelets.
Kamiyama M, Shirai T, Tamura S, Suzuki-Inoue K, Ehata S, Takahashi K, Miyazono K, Hayakawa Y, Sato T, Takeda K, Naguro I, Ichijo H
Kamiyama et al, 2017 accepted 28753204

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site10Frt
Map3k5
MGI:1346876		mitogen-activated protein kinase kinase kinase 510Map3k5<tm3.1Hijo> targeted mutation 3.1, Hidenori Ichijo
loxP phage P1 loxP10loxP
loxP phage P1 loxP10loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Saccharomyces cerevisiae FRT site, mouse Map3k5 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHidenori Ichijo (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data