Strain Data Sheet

RBRC10094

Strain Information

Image
BRC No.RBRC10094
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Irf3<tm2.2Ttg>
Former Common nameIRF3 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tadatsugu Taniguchi and Hideyuki Yanai, University of Tokyo in 2017. TT2 ES cells derived from C57BL/6 x CBA F1 were used. The neo gene was deleted by crossing the CAG-cre mice. Mice were backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic strain.
Strain descriptionIRF-3(Irf3:interferon regulatory factor)knockout mice. Homozygous mice are viable, susceptible to viral infection and exhibit lower serum interferon levels in response to viral infection. This strain has normal expression of Bcl2L12, an adjacent gene of the Irf-3.
Colony maintenance
References
Revisiting the role of IRF3 in inflammation and immunity by conditional and specifically targeted gene ablation in mice.
Yanai H, Chiba S, Hangai S, Kometani K, Inoue A, Kimura Y, Abe T, Kiyonari H, Nishio J, Taguchi-Atarashi N, Mizushima Y, Negishi H, Grosschedl R, Taniguchi T
Proc. Natl. Acad .Sci. USA, 115(20), 5253-5258 (2018). 29712834

Health Report

Examination Date / Room / Rack2024/12/09Room:3-2Rack:CSentinel mouse program
2024/09/09Room:3-2Rack:CSentinel mouse program
2024/06/10Room:3-2Rack:CSentinel mouse program
2024/03/11Room:3-2Rack:CSentinel mouse program
2023/12/11Room:3-2Rack:CSentinel mouse program
2023/09/11Room:3-2Rack:CSentinel mouse program
2023/06/13Room:3-2Rack:CSentinel mouse program
2023/03/13Room:3-2Rack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Irf3
MGI:1859179		interferon regulatory factor 37Irf3<tm2.2Ttg> targeted mutation 2.2, Tadatsugu Taniguchi
  • herpes simplex encephalitis, susceptibility to, 7(MedGEN)
    • loxP phage P1 loxP7loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, mouse Irf3 genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad .Sci. USA, 115(20), 5253-5258 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorTadatsugu Taniguchi (The University of Tokyo)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Nov 2020

    BRC mice in Publications

    No Data