Strain Data Sheet

RBRC10109

Strain Information

Image
BRC No.RBRC10109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Slc6a5<tm1.1(cre)Ksak>
Former Common nameGLYT2-Cre knockin mice
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeposited by Toshiakizu Kakizaki, Gunma University Graduate School of Medicine in 2017. RENKA ES cells derived from C57BL/6N was used. A neo gene was removed by crossing with a Flp mouse line. C57BL/6 genetic background.
Strain descriptionCre knock-in mouse at the translation initiation codon of the Slc6a5 (Glyt2) gene. Homozygous mice are lethal by 2 weeks after their birth.
Colony maintenance
References
A glycine transporter 2-Cre knock-in mouse line for glycinergic neuron-specific gene manipulation.
Kakizaki T, Sakagami H, Sakimura K, Yanagawa Y
IBRO Reports, 3, 9-16 (2017). 30135938

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:KSentinel mouse program
2024/10/21Room:3-CRack:KSentinel mouse program
2024/07/22Room:3-CRack:KSentinel mouse program
2024/04/22Room:3-CRack:KSentinel mouse program
2024/01/22Room:3-CRack:KSentinel mouse program
2023/10/23Room:3-CRack:KSentinel mouse program
2023/07/24Room:3-CRack:KSentinel mouse program
2023/04/24Room:3-CRack:KSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site7Frt
Slc6a5
MGI:105090		solute carrier family 6 (neurotransmitter transporter, glycine), member 57Slc6a5<tm1.1(cre)Ksak> targeted mutation 1.1, Kenji Sakimura
  • hyperekplexia 3(DisGeNET, MedGEN)
    • cre Phage P1 Cre recombinase7cre
    human granulocyte colony-stimulating factor gene polyA7

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 Cre, human granulocyte colony-stimulating factor gene polyA, yeast FRT (flipase recombination target) site, mouse Slc6a5 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. IBRO Reports, 3, 9-16 (2017). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without a permission of the DEPOSITOR scientist. The use of the BIOLOGICAL RESOURCE is only for academic researches but not for profit organizations.
    DepositorToshikazu Kakizaki (Gunma University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Dec 2019

    BRC mice in Publications

    Wei XP, Collie M, Dempsey B, Fortin G, Yackle K.
    A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing.
    Neuron 110(4) 644-657.e6(2022) 34998469