Strain Data Sheet
RBRC10142
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10142 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Gt(ROSA)26Sor<tm1(rtTA<*>M2)Jae> Col1a1<tm2(tetO-EWSR1/ATF1,-mCherry)Yasu> |
| Former Common name | Rosa26-M2rtTA/Col-tetO-EWS/ATF1 |
| H-2 Haplotype | |
| ES Cell line | KH2[F1:C57BL/6 x 129Sv] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shingo Komura and his colleagues in 2013 and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. KH2 ES cells derived from ((C57BL/6(f)× 129/SV(m))F1 were used. Mixed genetic background. |
| Strain description | Compound mouse line harboring the following two loci; 1, M2rtTA is knocked in at the Rosa26 locus, 2, tetO-EWSR1/ATF1-ires-mCherry is knocked in at the Col1a1 locus. |
| Colony maintenance | |
| References | EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice. Yamada K, Ohno T, Aoki H, Semi K, Watanabe A, Moritake H, Shiozawa S, Kunisada T, Kobayashi Y, Toguchida J, Shimizu K, Hara A, Yamada Y J. Clin. Invest., 123, 600-610 (2013). 23281395Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas. Komura S, Ito K, Ohta S, Ukai T, Kabata M, Itakura F, Semi K, Matsuda Y, Hashimoto K, Shibata H, Sone M, Jo N, Sekiguchi K, Ohno T, Akiyama H, Shimizu K, Woltjen K, Ozawa M, Toguchida J, Yamamoto T, Yamada Y Nat. Commun., 10(1):3999 (2019). 31488818 |
Health Report | |
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| Examination Date / Room / Rack | 2024/04/08Room:3-ARack:FSentinel mouse program 2024/01/09Room:3-ARack:FSentinel mouse program 2023/10/10Room:3-ARack:FSentinel mouse program 2023/07/10Room:3-ARack:FSentinel mouse program 2023/04/10Room:3-ARack:FSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| mCherry | Red Fluorescent Protein (Discosoma sp.) | 11 | mCherry | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1(rtTA<*>M2)Jae> | targeted mutation 1, Rudolf Jaenisch | |||
| Ires | internal ribosomal entry site (EMCV) | 6 | |||||
| hyg | hygromycin phasphotransferase (E. coli) | 6 | hyg | ||||
| puro | puromycin acyltransferase (Streptomyces alboniger) | 6 | puro | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Escherichia coli K-12 M2rtTA, Rabbit globin polyA, mouse PGK promoter, Streptomyces alboniger Puromycin resistance gene, mouse Rosa genomic DNA,adenovirus type 2 Splicing acceptor (SA), yeast Frt site, E. coli tetO (tetracycline operator), CMV promoter, human EWS cDNA, human ATF1 cDNA, FLAG tag(synthetic), Influenza virus A HA tag, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA, Escherichia coli K-12 hygromycin resistance gene, Escherichia coli ampicillin resistance gene, mouse Col1A1 genomic DNA |
| Research application | FLP/frt system Fluorescent Proteins/lacZ System Tet system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 10(1):3999 (2019).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Depositor | Yasuhiro Yamada (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |