Strain Data Sheet

RBRC10143

Strain Information

Image
BRC No.RBRC10143
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Sox10<tm1(cre/ERT2)Yasu>
Former Common nameSox10-CreERT2
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Shingo Komura and his colleagues in 2013 and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from ((C57BL/6 x 129S4/SvJae)F1) were used. Mixed genetic background.
Strain descriptionKnock-in mouse of CreERT2 to the 3’UTR of the Sox10 locus. Cre/loxP recombination can be induced by the administration of Tamoxifen.
Colony maintenance
References
Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. （in revision）

Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas.
Komura S, Ito K, Ohta S, Ukai T, Kabata M, Itakura F, Semi K, Matsuda Y, Hashimoto K, Shibata H, Sone M, Jo N, Sekiguchi K, Ohno T, Akiyama H, Shimizu K, Woltjen K, Ozawa M, Toguchida J, Yamamoto T, Yamada Y
Nat. Commun., 10(1):3999 (2019). 31488818

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bsd Aspergillus terreus blasticidin resistance gene (Bsd)15Bsd mouse phosphoglycerate kinase promoter (PGK promoter)
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)15ERT2
HBB2 rabbit beta-globin polyA15HBB2
Ires internal ribosomal entry site (EMCV)15
Sox10
MGI:98358		SRY (sex determining region Y)-box 1015Sox10<tm1(cre/ERT2)Yasu> targeted mutation 1, Yasuhiro Yamada
  • PCWH syndrome(DisGeNET, MedGEN)

  • Waardenburg syndrome type 4C(DisGeNET, MedGEN)

  • Waardenburg syndrome type 2E(MedGEN)
    • cre Phage P1 Cre recombinase15cre
    rox Bacteriophage D6 rox site15rox
    rox Bacteriophage D6 rox site15rox
    SV40 polyA signal15

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Sox10 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 10(1):3999 (2019). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorYasuhiro Yamada (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data