Strain Data Sheet
RBRC10143
Strain Information | |
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| Image | |
| BRC No. | RBRC10143 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Sox10<tm1(cre/ERT2)Yasu> |
| Former Common name | Sox10-CreERT2 |
| H-2 Haplotype | |
| ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shingo Komura and his colleagues in 2013 and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from ((C57BL/6 x 129S4/SvJae)F1) were used. Mixed genetic background. |
| Strain description | Knock-in mouse of CreERT2 to the 3’UTR of the Sox10 locus. Cre/loxP recombination can be induced by the administration of Tamoxifen. |
| Colony maintenance | |
| References | Nat. Commun., 10(1):3999 (2019). 31488818 Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. （in revision） |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sox10SRY (sex determining region Y)-box 1015Sox10<tm1(cre/ERT2)Yasu>targeted mutation 1, Yasuhiro Yamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter BsdAspergillus terreus blasticidin resistance gene (Bsd)15Bsdmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)15ERT2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA15HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)15 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase15cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site15rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site15rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal15 |
Ordering Information | |
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| Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Sox10 genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Depositor | Yasuhiro Yamada (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |