Strain Data Sheet
RBRC10145
Strain Information | |
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| Image | |
| BRC No. | RBRC10145 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Tppp3<tm1(EGFP)Yasu> |
| Former Common name | Tppp3-EGFP |
| H-2 Haplotype | |
| ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shingo Komura and his colleagues in 2013 and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from ((C57BL/6 x 129S4/SvJae)F1) were used. Mixed genetic background. |
| Strain description | Knock-in mouse of EGFP to the 3’UTR of the Tppp3 locus. |
| Colony maintenance | |
| References | Nat. Commun., 10(1):3999 (2019). 31488818 Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. （in revision） |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Tppp3tubulin polymerization-promoting protein family member 38Tppp3<tm1(EGFP)Yasu>targeted mutation 1, Yasuhiro Yamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter BsdAspergillus terreus blasticidin resistance gene (Bsd)8Bsdmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPGreen Fluorescent Protein (Aequorea victoria)8EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA8HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)8 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site8rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site8rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal8 |
Ordering Information | |
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| Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Aequorea victoria EGFP cDNA, Rabbit globin pA, D6 Bacteriophage rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Tppp3 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Depositor | Yasuhiro Yamada (Kyoto University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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