Strain Data Sheet

RBRC10147

Strain Information

Image
BRC No.RBRC10147
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Actb-PDHL/roGFP1)237Awolf
Former Common nameMito-roGFP mice(MRO237)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Alexnder M Wolf, G Nippon Medical School in 2012. Transgenic construct was injected into fertilized eggs derived from C57BL/6J. Mice were backcrossed to C57BL/6J for more than 10 generations. Mice were maintained as heterozygote.
Strain descriptionTransgenic mice expressing ratiometric redox-sensitive GFP (PDHL/roGFP1) in the mitochondria of several tissue under the control of chicken beta-actin promoter. Redox states can be monitored at liver and other tissues.
Colony maintenance
References
Real-time monitoring of oxidative stress in live mouse skin.
Wolf A M, Nishimaki K, Kamimura N, Ohta S
J. Invest. Dermatol., 134(6), 1701-1709 (2014). 24129062

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit beta-globin polyAUNHBB2
PDHA1 human Pyruvate dehydrogenase E1 subunit leader sequence (PHDL)UNPDHA1
roGFP1 redox-sensing Green Fluorescent Protein (Aequorea victoria)UNroGFP1 chicken beta-actin promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAChicken beta-Actin promoter, Human Pyruvate dehydrogenase E1 subunit leader sequence (PHDL) cDNA, Aquorea Victoria GFP variant (C48S/Q80R/S147C/Q204C) cDNA, Rabbit beta-globin PolyA signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAlexander M. Wolf (Nippon Medical School)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data