Strain Data Sheet

RBRC10149

Strain Information

Image
BRC No.RBRC10149
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Mafg-Nfe2l1)1Mym
Former Common nameMGRD-Nrf1-Tg mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masayuki Yamamoto, Tohoku University School of Medicine in 2012. DNA construct was injected to fertilized eggs derived from B6D2F1. Mice were backcrossed to C57BL/6J for 10 generations. Y chromosome is derived from C57BL/6J.
Strain descriptionTransgenic mice expressing mouse Nrf1 (Nfe2l1, nuclear factor, erythroid derived 2,-like 1) cDNA under the control of mouse Mafg promoter. Flag-tag was fused C-terminally to Nfe2l1 protein.
Colony maintenance
References
Transcription factor NF-E2-related factor 1 impairs glucose metabolism in mice.
Hirotsu Y, Higashi C, Fukutomi T, Katsuoka F, Tsujita T, Yagishita Y, Matsuyama Y, Motohashi H, Uruno A, Yamamoto M
Genes Cells. 19, 650-665 (2014). 25041126

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG FLAG tag (synthetic)UNFLAG
Mafg mouse Mafg pA and 3' regionUNMafg
Nfe2l1 nuclear factor, erythroid derived 2,-like 1UNNfe2l1 mouse Mafg promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal blood homeostasis(MP:0009642)

  • abnormal glucose homeostasis(MP:0002078)

  • abnormal pancreatic beta cell morphology(MP:0005217)

  • decreased body weight(MP:0001262)

  • decreased circulating ammonia level(MP:0030565)
    • more 7 phenotypes
  • enlarged pancreatic islets(MP:0009171)

  • homeostasis/metabolism phenotype(MP:0005376)

  • hyperglycemia(MP:0001559)

  • increased circulating insulin level(MP:0002079)

  • increased liver weight(MP:0002981)

  • increased susceptibility to diet-induced non-insulin dependent diabetes(MP:0020089)

  • insulin resistance(MP:0005331)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMus musculus Mafg regulatory domain (MGRD), Mus musculus Nfe2l1 (Nrf1) cDNA (3’ FLAG-tagged), Mus musculus Mafg pA and 3' region
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 19, 650-665 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For two years after deposition, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasayuki Yamamoto (Tohoku University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Wang Y, Snell A, Dyka FM, Colvin ER, Ildefonso C, Ash JD, Lobanova ES.
    Overexpression of Nfe2l1 increases proteasome activity and delays vision loss in a preclinical model of human blindness.
    Sci Adv 9(28) eadd5479(2023) 37450596