Strain Data Sheet

RBRC10161

Strain Information

Image
BRC No.RBRC10161
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(Myh6-Mas1)BChuan
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takashi Iwamoto, Chubu University in 2015. B6D2F1 fertilized eggs were usedfor microinjection. Mice were backcrossed to C57BL/6J three times.
Strain descriptionTransgenic line expressing murine Mas1 oncogene under the control of the murine Myh6 (myosin, heavy polypeptide 6) promoter. Two sublines (A, RBRC10160; B, RBRC10161) are available. The renin–angiotensin system (RAS) plays a pivotal role in the pathogenesis of cardiovascular diseases. G protein-coupled receptor Mas1 is a receptor for angiotensin (Ang) –(1-7), and ACE2/ Ang-(1-7)/MAS1 receptor axis have been shown to antagonize ACE1/Ang-II/AT1 receptor axis. However, this idea recently looks to become skeptical, and the physiological role of MAS1 in the hearts remains to be determined. B6;B6D2-Tg(Myh6-Mas1)AChuan (RBRC10160) and B6;B6D2-Tg(Myh6-Mas1)BChuan (RBRC10161) overexpress mouse MAS1 gene in cardiomyocytes and die earlier due to heart failure. B6;B6D2-Tg(Myh6-Mas1)BChuan (RBRC10161) mice express MAS1 gene much more highly than B6;B6D2-Tg(Myh6-Mas1)AChuan (RBRC10160) mice, and careful breeding is necessary.
Colony maintenance
References
Impairment of in vitro and in vivo heart function in angiotensin-(1-7) receptor MAS knockout mice.
Santos R A, Castro C H, Gava E, Pinheiro S V, Almeida A P, Paula R D, Cruz J S, Ramos A S, Rosa K T, Irigoyen M C, Bader M, Alenina N, Kitten G T, Ferreira A J
Hypertension, 47(5), 996-1002 (2006). 16567589

Inhibition of Mas G-protein signaling improves coronary blood flow, reduces myocardial infarct size, and provides long-term cardioprotection.
Zhang T, Li Z, Dang H, Chen R, Liaw C, Tran T A, Boatman P D, Connolly D T, Adams J W
Am. J. Physiol. Heart Circ. Physiol., 302(1), H299-311 (2012). 22003054

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (human)UNGH
Mas1 MAS1 oncogeneUNMas1 mouse Myh6 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAbeta-myosin heavy chain genomic DNA, Mus musculus alpha-myosin heavy chain promoter, Mus musculus MAS1 receptor cDNA, Homo sapiens Growh hormone polyA signal
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURSE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTakashi Iwamoto (Chubu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data