Strain Data Sheet
RBRC10162
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10162 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | R26-WntVis(Accession No.CDB0303K) |
| Former Common name | R26-WntVis(Accession No.CDB0303K) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Hisato Kondo, Faculty of Life Sciences, Kyoto Sangyo University and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology. C57BL/6 background. |
| Strain description | Wnt/beta-catenin signaling reporter mice. |
| Colony maintenance | |
| References | R26-WntVis reporter mice showing graded response to Wnt signal levels. Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H Genes Cells., 21(6):661-9 (2016). 27030109 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/01/07Room:3-BRack:ISentinel mouse program 2024/10/07Room:3-BRack:ISentinel mouse program 2024/07/08Room:3-BRack:IAdditional tests 2024/04/08Room:3-BRack:ISentinel mouse program 2024/01/09Room:3-BRack:ISentinel mouse program 2023/10/10Room:3-BRack:ISentinel mouse program 2023/07/10Room:3-BRack:ISentinel mouse program 2023/04/10Room:3-BRack:ISentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 6 | EGFP | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1.1(TCF/Lef-H2B/EGFP)Knd> | targeted mutation 1.1, Hisato Kondo | |||
| H2B | human histone H2B cDNA | 6 | H2B | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | |||
| HBB2 | rabbit beta-globin polyA | 6 | HBB2 | ||||
| SV40 polyA signal | 6 | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | mouse TCF/Lef binding site, herpes simplex virus thymidine kinase promoter (HSV tk promoter), human H2B cDNA, Jellyfish EGFP cDNA, SV40 polyA signal, rabbit b-globin pA, yeast FRT (flippase recombination target) site, mouse Gt(ROSA)26Sor region genomic DNA |
| Research application | FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 21(6):661-9 (2016). (1) When publishing the research results obtained by the use of the BIOLOGICAL RESOURCE, the RECIPIENT shall refer to the Accession No. CDB0303K and URL (https://large.riken.jp/distribution/mutant-list.html) in the manuscript. (2) The RECIPIENT shall inform the DEPOSITOR (mutant.bdr@riken.jp, kondohh@cc.kyoto-su.ac.jp, and takemoto.tatsuya@tokushima-u.ac.jp) of any publications with the use of the BIOLOGICAL RESOURCE. |
| Depositor | RIKEN CDB (RIKEN CDB) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
| No Data |