Strain Data Sheet

RBRC10167

Strain Information

Image
BRC No.RBRC10167
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gprc5c<tm1Yhir>
Former Common nameGPRC5C-deficient, (Acc. No. CDB0485K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentGenerated by RIKEN CDB(Acc. No. CDB0480K). TT2 ES cell line [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. Exon 2 was replaced with a fragment lacZ-neo. C57BL/6J congenic strain.
Strain descriptionGprc5c(G protein-coupled receptor, family C, group 5, member C)knockout mice. Mutants exhibit no obvious phenotype.
Colony maintenance
References
Comparative characterization of GPRC5B and GPRC5CLacZ knockin mice; behavioral abnormalities in GPRC5B-deficient mice.
Sano T, Kim Y J, Oshima E, Shimizu C, Kiyonari H, Abe T, Higashi H, Yamada K, Hirabayashi Y
Biochem. Biophys. Res. Commun., 412(3), 460-465 (2011). 21840300

Health Report

Examination Date / Room / Rack2024/01/09Room:3-ARack:GSentinel mouse program
2023/10/16Room:3-ARack:GAdditional tests
2023/10/10Room:3-ARack:GSentinel mouse program
2023/07/10Room:3-ARack:GSentinel mouse program
2023/04/10Room:3-ARack:GSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site11Frt
Frt yeast FRT (flippase recombination target) site11Frt
Gprc5c
MGI:1917605		G protein-coupled receptor, family C, group 5, member C11Gprc5c<tm1Yhir>
MGI:5293388		targeted mutation 1, Yoshio Hirabayashi
lacZ beta-galactosidase (E. coli)11
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA7

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • behavior/neurological phenotype(MP:0005386)

  • decreased mean corpuscular hemoglobin concentration(MP:0005642)

  • increased basophil cell number(MP:0002606)

  • increased circulating alkaline phosphatase level(MP:0002968)

  • increased circulating lactate dehydrogenase level(MP:0011971)
    • more 3 phenotypes
  • increased mean corpuscular volume(MP:0002590)

  • nervous system phenotype(MP:0003631)

  • reticulocytosis(MP:0002640)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 lox71 site, Phage P1 loxP site, E. coli lacZ gene, yeast FRT (flippase recombination target) sites, Mouse Phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Gprc5c genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorYoshio Hirabayashi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data