Strain Data Sheet

RBRC10171

Strain Information

Image
BRC No.RBRC10171
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm3(rtTA3)Yasu> Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>
Former Common nameRosa26-LSL-rtTA3/ Col-tetO-OSKM
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Hirofumi Shibata and his colleagues in 2015, and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1 were used. Mixed genetic background.
Strain descriptionCompound mouse line harboring the following two loci; 1, loxP-Pgk-neo-loxP-rtTA3 is knocked in at the Rosa26 locus, 2, tetO-OSKM-ires-mCherry is knocked in at the Col1a1 locus. OSKM stands for Oct3/4, Sox2, Klf4 and c-Myc.
Colony maintenance
References
In vivo reprogramming drives Kras-induced cancer development.
Shibata H, Komura S, Yamada Y, Sankoda N, Tanaka A, Ukai T, Kabata M, Sakurai S, Kuze B, Woltjen K, Haga H, Ito Y, Kawaguchi Y, Yamamoto T, Yamada Y
Nature Commun., 9, 2081 (2018). 29802314

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Col1a1
MGI:88467		collagen, type I, alpha 111Col1a1<tm4(tetO-OSKM,-mCherry)Yasu> targeted mutation 4, Yasuhiro Yamada
  • Caffey disease(MedGEN)

  • combined osteogenesis imperfecta and Ehlers-Danlos syndrome 1(MedGEN)

  • Ehlers-Danlos syndrome, arthrochalasis type(MedGEN)
    • more 6 Diseases
  • osteogenesis imperfecta type 1(DisGeNET, MedGEN)

  • osteogenesis imperfecta type 2(DisGeNET, MedGEN)

  • osteogenesis imperfecta type 3(DisGeNET, MedGEN)

  • osteogenesis imperfecta type 4(DisGeNET, MedGEN)

  • osteogenesis imperfecta(DisGeNET)

  • osteoporosis(MedGEN)
    • Gt(ROSA)26Sor
    MGI:104735    		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm3(rtTA3)Yasu> targeted mutation 3, Yasuhiro Yamada
    E2A Equine rhinitis A virus (ERAV) 2A peptideE2A
    Frt yeast FRT (flippase recombination target) siteFrt
    HBB2 Rabbit globin polyAHBB2
    Hyg hygromycin phasphotransferase (E. coli)Hyg
    Ires internal ribosomal entry site (EMCV)
    P2A porcine teschovirus-1 2A peptideP2A
    SA Splice acceptor (adenovirus)SA
    T2A Thosea asigna virus 2A peptide (insect)T2A
    loxP phage P1 loxPloxP
    loxP phage P1 loxPloxP
    mCherry Red Fluorescent Protein (Discosoma sp.)mCherry
    neo neomycin resistance gene (Klebsiella pneumoniae) mouse phosphoglycerate kinase promoter (PGK promoter)
    SV40 polyA

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus type2 Splicing acceptor (SA) [Rosa26, Col1a1 locus], P1 Bacteriophage loxP sites, mouse Pgk1 promoter, Klebsiella pneumoniae (Bacterial transposon Tn5) Neomycin registance gene (Neo), SV40 pA, Escherichia coli K-12 strain derivative rtTA3, Escherichia coli K-12 strain derivative Hygromycin registance gene, CMV promoter, E. coli tetO (tetracycline operator), yeast Frt site, Escherichia coli ampicillin registance gene, mouse Oct3/4, mouse Sox2, mouse Klf4, mouse c-Myc, Porcineteschovirus-1 P2A,Thoseaasignavirus T2A,Equine rhinitis A virus E2A, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Commun., 9, 2081 (2018). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorYasuhiro Yamada (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: FP license of TBUSA
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data