Strain Data Sheet

RBRC10172

Strain Information

Image
BRC No.RBRC10172
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Sftpc<tm1(cre/ERT2)Yasu>
Former Common nameSftpC-CreERT2
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Hirofumi Shibata and his colleagues in 2015, and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1 were used. Mixed genetic background.
Strain descriptionKnock-in mouse of CreERT2 at the translation initiation codon of the SftpC gene. Cre/loxP recombination can be induced by the administration of Tamoxifen.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/11/18Room:4-ARack:FSentinel mouse program
2024/08/19Room:4-ARack:FSentinel mouse program
2024/05/20Room:4-ARack:FSentinel mouse program
2024/02/19Room:4-ARack:FSentinel mouse program
2023/11/20Room:4-ARack:FSentinel mouse program
2023/08/28Room:4-ARack:FAdditional tests
2023/08/21Room:4-ARack:FSentinel mouse program
2023/06/19Room:4-ARack:FAdditional tests
2023/05/22Room:4-ARack:FSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bsd Aspergillus terreus blasticidin resistance gene (Bsd)14Bsd
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)14ERT2
HBB2 rabbit beta-globin polyA14HBB2
Sftpc
MGI:109517		surfactant associated protein C14Sftpc<tm1(cre/ERT2)Yasu> targeted mutation 1, Yasuhiro Yamada
  • surfactant metabolism dysfunction, pulmonary, 2(MedGEN)
    • cre Phage P1 Cre recombinase14cre
    SV40 polyA signal14

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP1 Bacteriophage Cre recomibinase, human Estrogen receptor binding domain (ERT2)cDNA, Rabbit globin pA(CreERT2), D6 Bacteriophage rox site, mouse PGK promoter, Aspergillus terreus Blasticidin S deaminase gene (Bsd), Simian virus (SV40) pA, mouse Sftpc genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEVELOPER. The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
    DepositorYasuhiro Yamada (Kyoto University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data