Strain Data Sheet
RBRC10180
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10180 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Mboat4<tm1Shi> |
| Former Common name | GOAT-knockout mouse, GOAT-KO |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | BRUCE-4 ES cell line (derived from C57BL/6J mouse line) was used for homologous recombination. The mice were generated by Dr. Tomohiko Okuda et al. in Aburahi Laboratories of SHIONOGI & CO., LTD. in 2010. Chimeras generated using these ES cells were bred with C57BL/6JJcl (CLEA Japan, Inc.). After confirmation of germ-line transmission, homozygous knockout mice were generated by inbreeding of heterozygous mice. B6-Mboat4tm1Shi homozygotes were maintained via incrossing. The engineered mutation was transmitted to the progeny with the expected Mendelian ratio and develop normally. |
| Strain description | A null KO mouse of the Mboat4 gene encoding ghrelin O-acyltransferase (GOAT). Exon1 of the gene was replaced with a PGK-Neo cassette by homologous recombination in ES cells. Homozygous KO mice show no acylated ghrelin. This model mouse can be utilised for reserches of diseases, inter alia, metabolic syndrome. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | Ghrelin O-acyltransferase knockout mice show resistance to obesity when fed high-sucrose diet. Kouno T, Akiyama N, Ito T, Okuda T, Nanchi I, Notoya M, Oka S, Yukioka H J. Endocrinol., 228(2), 115-125 (2016). 26645250Reduced intake of carbohydrate prevents the development of obesity and impaired glucose metabolism in ghrelin O-acyltransferase knockout mice. Kouno T, Akiyama N, Fujieda K, Nanchi I, Okuda T, Iwasaki T, Oka S, Yukioka H Peptides., ;86, 145-152 (2016). 27816752 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Mboat4 MGI:2685017 | membrane bound O-acyltransferase domain containing 4 | 8 | Mboat4<tm1Shi> | targeted mutation 1, Shionogi and Company, Ltd | |||
| loxP | phage P1 loxP | 8 | loxP | ||||
| loxP | phage P1 loxP | 8 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 8 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | more 5 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | P1 phage loxP sites, Mouse Phosphoglycerate kinase (PGK) promoter, E.coli Neomycin resistant gene, SV40 polyA additional signal, mouse Mboat4 genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J .Endocrinol., 228(2), 115-125 (2016).1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. 2. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit academic institution for a not-for-profit academic research. 3. The Recipient must not provide any third party with the BIOLOGICAL RESOURCE without prior DEPOSITOR’s permission. |
| Depositor | Hirohide Nambu (Shionogi & Co., Ltd) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
|---|
| No Data |