Strain Data Sheet

RBRC10182

Strain Information

Image
BRC No.RBRC10182
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pou3f2<tm1.1(dGQP)Lmame>
Former Common namePou3f2 delta GQP
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shintaroh Ueda and Makoto Nasu, The University of Tokyo in 2003. Targeting vector was introduced into the E14-1 ES cells. Cre expression plasmid was further introduced into positive clones to remove the neo cassette. Mice were backcrossed to the C57BL/6J for more than 7 generations. C57BL/6J congenic line.
Strain descriptionPou3f2 mutant mice in which homopolymeric amino acid repeats of Pou3f2 specific to mammalian species were deleted in the mouse Pou3f2 gene. Poor maternal behavior with decreased expression of dopamine and serotonin synthesizing enzymes.
Colony maintenance
References
Mammalian-specific sequences in pou3f2 contribute to maternal behavior.
Nasu M, Yada S, Igarashi A, Sutoo D, Akiyama K, Ito M, Yoshida N, Ueda S
Genome Biol. Evol., 6(5):1145-1156 (2014). 24709564

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pou3f2
MGI:101895		POU domain, class 3, transcription factor 24Pou3f2<tm1.1(dGQP)Lmame> targeted mutation 1.1, Shintaroh Ueda
loxP phage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal nursing(MP:0001382)

  • decreased dopamine level(MP:0005643)

  • decreased serotonin level(MP:0010070)

  • postnatal lethality, complete penetrance(MP:0011085)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Pou3f2 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genome Biology and Evolution 6(5):1145-1156 (2014) doi:10.1093/gbe/evu072In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShintaroh Ueda (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data