Strain Data Sheet
RBRC10188
Strain Information | |
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| Image | |
| BRC No. | RBRC10188 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Gt(ROSA)26Sor<tm1(CAG-3XFLAG/dCas9,-EGFP)Hfuj> |
| Former Common name | 3×FLAG-dCas9/CTV: neo (+), GFP (+) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Hodaka Fujii/Osaka University and Ichiro Taniuchi/RIKEN in 2016. B6JN/1 ES cells [(C57BL/6JJcl x C57BL/6NJcl)F1] were used. C57BL/6 background. This line has been maintained by crossing between heterozygotes and C57BL/6. No obvious phenotype was observed. |
| Strain description | Knockin mice for Cre-dependent expression of dCas9 and EGFP. DNA fragment of CAG-loxP-Neo-Stop-loxP-3xFLAG-dCas9-Frt-IRES-EGFP-Frt-pA was knocked in at the Rosa26 locus. |
| Colony maintenance | |
| References | Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis. Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H Genes Cells. 23(4):318-325 (2018). 29480524 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 6 | EGFP | ||||
| FLAG | 3xFLAG tag (synthetic) | 6 | FLAG | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1(CAG-3XFLAG/dCas9,-EGFP)Hfuj> | targeted mutation 1, Hodaka Fujii | |||
| Ires | internal ribosomal entry site (EMCV) | 6 | |||||
| dCas9 | Streptococcus pyogenes Cas9 mutant | 6 | dCas9 | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene | 6 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||
| SV40 polyA | 6 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | adenoviral splice acceptor (SA), CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neomycin resistance gene, tk polyA, yeast His3 gene, Synthetic 3xFLAG tag, Streptococcus pyogenes Cas9 mutant (dCas9), yeast FRT (flipase recombination target) sites, ECMV internal ribosomal entry site (ires), Aequorea victoria green fluorescent protein (GFP) cDNA, bovine GH polyA, mouse Rosa26 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 23(4):318-325 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Hodaka Fujii (Hirosaki University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Oct 2019 |
BRC mice in Publications |
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| No Data |