Strain Data Sheet

RBRC10190

Strain Information

Image
BRC No.RBRC10190
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1.2(CAG-3XFLAG/dCas9)Hfuj>
Former Common name3×FLAG-dCas9/CTV
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hodaka Fujii/Osaka University and Ichiro Taniuchi/RIKEN in 2016. B6JN/1 ES cells [(C57BL/6JJcl x C57BL/6NJcl)F1] were used. C57BL/6 background. This line has been maintained by crossing between heterozygotes and C57BL/6. No obvious phenotype was observed.
Strain descriptionKnockin mice expressing dCas9. Parental knockin mice were generated by targeting DNA fragment, CAG-loxP-Neo-Stop-loxP-3xFLAG-dCas9-Frt-IRES-EGFP-Frt-pA, to the Rosa26 locus. The mice were further crossed with CAG-Cre mice, and then with CAG-Flpe, to remove loxP-Neo-Stop-loxP and Frt-IRES-EGFP-Frt cassettes.
Colony maintenance
References
Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.
Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H
Genes Cells. 23(4):318-325 (2018). 29480524

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG 3xFLAG tag (synthetic)6FLAG CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
Frt yeast FRT (flippase recombination target) site6Frt
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.2(CAG-3XFLAG/dCas9)Hfuj> targeted mutation 1.2 Hodaka Fujii
dCas9 Streptococcus pyogenes Cas9 mutant6dCas9
loxP phage P1 loxP6loxP
SV40 polyA6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenoviral splice acceptor (SA), CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP site, Synthetic 3xFLAG tag, Streptococcus pyogenes Cas9 mutant (dCas9), yeast FRT (flipase recombination target) site, SV40 polyA, mouse Rosa26 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 23(4):318-325 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHodaka Fujii (Hirosaki University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Oct 2019

    BRC mice in Publications

    No Data