Strain Data Sheet

RBRC10192

Strain Information

Image
BRC No.RBRC10192
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Sema4D<tm1In>
Former Common nameSema4DKO
H-2 Haplotype
ES Cell lineEB3 [129/Ola]
Background strain
Appearance
Strain developmentDeveloped by Shinobu Inagaki, Osaka University Graduate School of Medicine, Division of Health Sciences. EB3 ES cells derived from 129P2/OlaHsd were used. Mixed genetic background with C57BL/6.
Strain descriptionSema4d [sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D] knockout mice. IRES-tauLacZ-Neo-pA fragment was inserted between exon 1 and exon 2.
Colony maintenance
ReferencesJ. Neurosci. Res., 87, 2833-2841 (2009). 19472224

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sema4dsema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D13Sema4d<tm1In>targeted mutation 1, Shinobu Inagaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)13

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene13mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
taubovine tau protein cDNA13tau

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal13

Ordering Information

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bovine tau cDNA, E. coli LacZ, SV40 polyA signal, mouse phosphoglycerate kinase promoter (Pgk promoter), E. coli Neomycine resistant gene, mouse Sema4D genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci. Res., 87, 2833-2841 (2009).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorDrs. Tatsuo Furuyama and Shinobu Inagaki (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data