Strain Data Sheet

RBRC10195

Strain Information

Image
BRC No.RBRC10195
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameBALB/c-Ffar4<tm1Kync>
Former Common nameGPR120KO mouse
H-2 Haplotype
ES Cell line
Background strainBALB/cCrSlc
Appearance
Strain developmentDeposited by Kazuo Inoue, Graduate School of Agriculture, Kyoto University. ES cells derived from BALB/c genetic background were used. BALB/c genetic background.
Strain descriptionFfar4, free fatty acid receptor (別名Gpr120, G-protein coupled receptor 120) knockout mice. Exon containing the translation initiation codon was replaced with the Neo gene. BALB/c genetic background. Homozygous mutants are viable and fertile and shown no gross abnormality.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2023/11/27Room:4-BRack:ESentinel mouse program
2023/08/30Room:4-BRack:EAdditional tests
2023/08/28Room:4-BRack:ESentinel mouse program
2023/05/29Room:4-BRack:ESentinel mouse program
2023/03/22Room:4-BRack:EAdditional tests

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ffar4
MGI:2147577		free fatty acid receptor 419Ffar4<tm1Kync> targeted mutation 1, Shigenobu Matsumura
loxP phage P1 loxP19loxP
loxP phage P1 loxP19loxP
neo neomycin resistance gene (E. coli)19 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEnterobacteria phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, mouse Pgk1 polyA, mouse Ffar4(GPR120) genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKazuo Inoue (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data