Strain Data Sheet

RBRC10196

Strain Information

Image
BRC No.RBRC10196
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(CAG-Tysnd1)Okazk>
Former Common nameTysnd1 Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeposited by Yasushi Okazaki, Saitama Medical University. C57BL/6 genetic background. ES cells derived from C57BL/6N were used. Mice were crossed with C57BL/6J.
Strain descriptionKockin mice overexpressing Tysnd1 (trypsin domain containing 1). CAG-Tysnd1 construct was knocked in at the ROSA26 locus.
Colony maintenance
References
Novel peroxisomal protease Tysnd1 processes PTS1- and PTS2-containing enzymes involved in beta-oxidation of fatty acids.
Kurochkin I V, Mizuno Y, Konagaya A, Sakaki Y, Schonbach C, Okazaki Y
EMBO J., 26(3), 835-845 (2007). 17255948

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-Tysnd1)Okazk> targeted mutation 1, Yasushi Okazaki
SA Splice acceptor (adenovirus)6SA
Tysnd1
MGI:1919017		trypsin domain containing 16Tysnd1 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus splice acceptor site, Phage P1 loxP sites, E. coli neo, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mouse Tysnd1 cDNA, mouse Tysnd1 poly A tail, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 26(3), 835-845 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Use the following sentence, “We akcnowledge Y. Okazaki, C. Schönbach and Y. Mizuno for providing the Tysnd1 TG mice.”.
    DepositorYasushi Okazaki (Saitama Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data