Strain Data Sheet

RBRC10202

Strain Information

Image
BRC No.RBRC10202
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameCB17.Cg-Sema4D<tm1In>
Former Common nameCB17-Sema4D-KO
H-2 Haplotype
ES Cell lineEB3 [129/Ola]
Background strain
Appearance
Strain developmentDeveloped by Shinobu Inagaki, Osaka University Graduate School of Medicine, Division of Health Sciences. EB3 ES cells derived from 129P2/OlaHsd were used. This strain was backcrossed to C.B-17/lcr-+/+Jcl for 10 generations.
Strain descriptionSema4d [sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D] knockout mice. IRES-tauLacZ-Neo-pA fragment was inserted between exon 1 and exon 2. C.B-17/lcr congenic strain.
Colony maintenance
References
Sema4D deficiency results in an increase in the number of oligodendrocytes in healthy and injured mouse brains.
Taniguchi Y, Amazaki M, Furuyama T, Yamaguchi W, Takahara M, Saino O, Wada T, Niwa H, Tashiro F, Miyazaki J, Kogo M, Matsuyama T, Inagaki S
J. Neurosci. Res., 87, 2833-2841 (2009). 19472224

Effect of Sema4D on microglial function in middle cerebral artery occlusion mice.
Sawano T, Watanabe F, Ishiguchi M, Doe N, Furuyama T, Inagaki S
Glia, 6, 2249-2259 (2015). 26202989

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ires internal ribosomal entry site (EMCV)13
Sema4d
MGI:109244		sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D13Sema4d<tm1In> targeted mutation 1, Shinobu Inagaki
lacZ beta-galactosidase (E. coli)13
neo neomycin resistance gene13 mouse phosphoglycerate kinase promoter (PGK promoter)
tau bovine tau protein cDNA13tau
SV40 polyA signal13

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal glial cell apoptosis(MP:0010170)

  • abnormal microglial cell activation(MP:0010762)

  • abnormal nitric oxide homeostasis(MP:0003957)

  • decreased microglial cell number(MP:0011982)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bovine tau cDNA, E. coli LacZ, SV40 polyA signal, mouse phosphoglycerate kinase promoter (Pgk promoter), E. coli Neomycine resistant gene, mouse Sema4D genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci. Res., 87, 2833-2841 (2009). Glia, 6, 2249-2259 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 　RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorDrs. Tatsuo Furuyama and Shinobu Inagaki (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data