Strain Data Sheet

RBRC10208

Strain Information

Image
BRC No.RBRC10208
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Src<tm1(S75D)Goka>
Former Common nameSrcS75D, SrcSD
H-2 Haplotype
ES Cell lineCCE [129/Sv]
Background strain
Appearance
Strain developmentDeveloped by Goro Kato, University of Yamanashi in 2001. CCE ES cells derived from 129/SvEv were used. C57BL/6 congenic strain by backcrossing to C57BL/6N for 6 generations.
Strain descriptionMutant mice in which a point mutation in the Src gene was introduced at Serine 75 to convert it to Aspartic acid (S75D).
Colony maintenance
References
Production of mouse ES cells homozygous for Cdk5-phosphorylated site mutation in c-Src alleles.
Kato G, Maeda S
J. Biochem., 133, 563-569 (2003). 12801906

A Ser75-to-Asp phospho-mimicking mutation in Src accelerates ageing-related loss of retinal ganglion cells in mice.
Kashiwagi K, Ito S, Maeda S, Kato G
Sci. Rep., 7, 16779 (2017). 29196663

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SrcRous sarcoma oncogene2Srctargeted mutation 1, Goro Kato
  • colorectal cancer(MedGEN)

  • thrombocytopenia 6(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased retina ganglion cell number(MP:0006309)

  • decreased susceptibility to induced retina damage(MP:0003455)

  • neurodegeneration(MP:0002229)

  • retina ganglion cell degeneration(MP:0008067)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Src genomic DNA (S75D mutant)
    Research application
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorGoro Kato (University of Yamanashi)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data