Strain Data Sheet

RBRC10213

Strain Information

Image
BRC No.RBRC10213
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gcfc2<tm1.1Hko>
Former Common nameGcfc2 cond.
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN, in 2016. M1(B6/129 ) ES cells were used. This strain was backcrossed to C57BL/6J twice.
Strain descriptionFloxed Gcfc2 (GC-rich sequence DNA binding factor 2) mice. A pair of loxP flanks Exon 1 and 2.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
Gcfc2
MGI:2141656		GC-rich sequence DNA binding factor 26Gcfc2<tm1.1Hko> targeted mutation 1.1, Haruhiko Koseki
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) site, phage P1 loxP sites, mouse Gcfc2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data