Strain Data Sheet

RBRC10217

Strain Information

Image
BRC No.RBRC10217
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Sf3b1<tm1Hko>
Former Common nameSap155
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki and Kyoichi isono, RIKEN, in 2003. M1(B6/129 ) ES cells were used. This strain was backcrossed to C57BL/6J twice.
Strain descriptionFloxed Zfp593 (zinc finger protein 593) mice. Four exons were replaced with a neo cassette.
Colony maintenance
References
Mammalian polycomb-mediated repression of Hox genes requires the essential spliceosomal protein Sf3b1.
Isono K, Mizutani-Koseki Y, Komori T, Schmidt-Zachmann M S, Koseki H
Gene&Development 19:536-541(2005). 15741318

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sf3b1
MGI:1932339		splicing factor 3b, subunit 11Sf3b1<tm1Hko> targeted mutation 1, Haruhiko Koseki
  • myelodysplastic syndrome(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)1 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • cervical vertebral transformation(MP:0004615)

  • embryonic lethality before implantation, complete penetrance(MP:0011094)

  • lumbar vertebral transformation(MP:0004616)

  • thoracic vertebral transformation(MP:0004618)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, poly A, mouse Sf3b1 genomic DNA
    Research application
    Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data