Strain Data Sheet

RBRC10222

Strain Information

Image
BRC No.RBRC10222
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameBALB/c-S100a9<em1Fjw>
Former Common nameMRP14-KO BALB/cA mice
H-2 Haplotype
ES Cell line
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Wataru Fujii and Yasuyuki Goto, The University of Tokyo in 2017. Fertilized eggs derived from BALB/cAJcl was used. BALB/cAJcl genetic background.
Strain descriptionS100a9 [S100 calcium binding protein A9 (calgranulin B)] mutant mice generated by the CRISPR/Cas9. 338 bp genomic fragment covering exon 2 was deleted. Deleted genomic sequence, (Chr 3: 90695035 to 90695372 (GRCm38.p4))TGGCCAGAGACTAGGTCAGGGAAGCTTGGTATCCCATTCTTTTATTTGTAACTTTTGTTTCCCCTATTTTCAAAGAAGAGCAAGAAGATGGCCAACAAAGCACCTTCTCAGATGGAGCGCAGCATAACCACCATCATCGACACCTTCCATCAATACTCTAGGAAGGAAGGACACCCTGACACCCTGAGCAAGAAGGAATTCAGACAAATGGTGGAAGCACAGTTGGCAACCTTTATGAAGGTGAGGCTTGGCTTGTGAGAGAAGAGGGTGCAATCTGCAGCAGTCTGAGAACAAATTCTAGCACCGGGGGCAGTAGGGCTCTGCCATTAGAGGCACTC
Colony maintenance
References
MRP14 is dispensable for LPS-induced shock in BALB/c mice.
Mizobuchi H, Fujii W, Ishizuka K, Wang Y, Watanabe S, Sanjoba C, Matsumoto Y, Goto Y
Immunol. Lett., 194, 13-20 (2017). 29253495

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
S100a9
MGI:1338947		S100 calcium binding protein A9 (calgranulin B)3S100a9<em1Fjw> endonuclease-mediated mutation 1, Wataru Fujii

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased neutrophil cell number(MP:0000222)

  • increased lymphocyte cell number(MP:0005013)

  • increased myeloid cell number in bone marrow(MP:0013415)

  • increased tumor necrosis factor secretion(MP:0008560)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunol. Lett., 194, 13-20 (2017).
    DepositorYasuyuki Goto (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data