Strain Data Sheet

RBRC10232

update : 2025-03-19

Strain Information
Image
BRC No.	RBRC10232
Type	Targeted Mutation Congenic
Species	Mus musculus
Strain name	B6.Cg-Reck<tm1Noda>
Former Common name	Reck R2neo, Reck <Low>, Reck <Hypo>
H-2 Haplotype
ES Cell line	TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development	Deposited and developed by Makoto Noda, Kyoto University Graduate School of Medicine, in collaboration with RIKEN CDB. TT2 ES cells were used. This strain was backcrossed to C57BL/6J for 13 generations. C57BL/6 congenic strain.
Strain description	Reck (reversion-inducing-cysteine-rich protein with kazal motifs) mutant mice. Exon 2 was floxed and FRT-pgk-neo-FRT was inserted at Intron 1. FRT-pgk-neo-FRT was not removed yet. Expression of RECK in homozyogous mice is less than 50% of that of wildtype mice.
Colony maintenance
References	Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice. Chandana E P, Maeda Y, Ueda A, Kiyonari H, Oshima N, Yamamoto M, Kondo S, Oh J, Takahashi R, Yoshida Y, Kawashima S, Alexander D B, Kitayama H, Takahashi C, Tabata Y, Matsuzaki T, Noda M BMC Dev. Biol., 10, 84 (2010). 20691046 The transformation suppressor gene Reck is required for postaxial patterning in mouse forelimbs. Yamamoto M, Matsuzaki T, Takahashi R, Adachi E, Maeda Y, Yamaguchi S, Kitayama H, Echizenya M, Morioka Y, Alexander D B, Yagi T, Itohara S, Nakamura T, Akiyama H, Noda M Biol. Open, 1(5),458-466 (2012). 23213437

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Frt	yeast FRT (flippase recombination target) site	4	Frt
Frt	yeast FRT (flippase recombination target) site	4	Frt
Reck MGI:1855698	reversion-inducing-cysteine-rich protein with kazal motifs	4	Reck<tm1Noda>	targeted mutation 1, Makoto Noda
loxP	phage P1 loxP	4	loxP
loxP	phage P1 loxP	4	loxP
neo	neomycin resistance gene	4				mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype
Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information
Donor DNA	Yeast flippase recombination target sites (FRT), Mouse Phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, 3’-UTR, Bacteriophage P1 loxP sites, mouse Reck genomic DNA
Research application	Cre/loxP system FLP/frt system
Specific Term and Conditions	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Open, 1(5),458-466 (2012).
Depositor	Makoto Noda (Kyoto University)
Strain Status	Frozen sperm
Strain Availability	Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month)
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data