Strain Data Sheet

RBRC10233

Strain Information

Image
BRC No.RBRC10233
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Reck<tm2.2Noda>
Former Common nameReck delta
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited and developed by Makoto Noda, Kyoto University Graduate School of Medicine. E14(129P2/OlaHsd)ES cells were used. This strain was backcrossed to C57BL/6J for 13 generations. C57BL/6 congenic strain.
Strain descriptionReck (reversion-inducing-cysteine-rich protein with kazal motifs) mutant mice. Exon 1 was replaced with a loxP site. Neo fragment was removed by crossing with EIIa-cre. Homozygous mutants are embryonically lethal at E9.5-E10.5.
Colony maintenance
References
Critical roles for murine Reck in the regulation of vascular patterning and stabilization.
de Almeida G M, Yamamoto M, Morioka Y, Ogawa S, Matsuzaki T, Noda M
Sci. Rep., 5:17860 (2015). 26658478

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Reck
MGI:1855698		reversion-inducing-cysteine-rich protein with kazal motifs4Reck<tm2.2Noda>
MGI:5428466		targeted mutation 2.2, Makoto Noda
loxP phage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal apical ectodermal ridge morphology(MP:0001676)

  • abnormal digit morphology(MP:0002110)

  • abnormal forelimb morphology(MP:0000550)

  • abnormal limb bud morphology(MP:0005650)

  • abnormal limb morphology(MP:0002109)
    • more 4 phenotypes
  • absent ulna(MP:0004360)

  • embryonic lethality during organogenesis(MP:0006207)

  • limbs/digits/tail phenotype(MP:0005371)

  • skeleton phenotype(MP:0005390)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP site, mouse Reck genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Almeida et al. Sci Rep 5:17860 (2015).
    DepositorMakoto Noda (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data