Strain Data Sheet

RBRC10238

Strain Information

Image
BRC No.RBRC10238
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tg(Cryd1/Cryaa-Sfrp2)1Jmca
Former Common nameSfrp2 lens (M2)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentTransgenic construct was injected into BCBF1 (C57/BL6xCBA) embryos. Generated by John McAvoy laboratory in the University of Sydney.After backcrossing to C57/BL6 line for a few generations, the line has been maintained as inbred.Small lens formation. Cataract development.Mice can be maintained as heterozygous or homozygous.
Strain descriptionTransgenic mouse line with Sfrp2 (secreted frizzled related protein-2) overexpression in lens.
Colony maintenance
References
Wnt signaling is required for organization of the lens fiber cell cytoskeleton and development of lens three-dimensional architecture.
Chen Y, Stump R J, Lovicu F J, Shimono A, McAvoy J W
Dev. Biol., 324, 161-176 (2008). 18824165

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (human)UNGH
Sfrp2 secreted frizzled-related protein 2UNSfrp2 Chicken delta 1-crystallin (ASL1) enhancer and promoter, Mouse alpha A-crystallin (Cryaa) promoter, Rabbit beta-globin intron

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal lens development(MP:0005545)

  • abnormal lens epithelium morphology(MP:0003237)

  • abnormal lens fiber morphology(MP:0002840)

  • abnormal lens morphology(MP:0001303)

  • abnormal microtubule cytoskeleton morphology(MP:0020850)
    • more 6 phenotypes
  • disorganized lens bow(MP:0003847)

  • disorganized secondary lens fibers(MP:0002579)

  • microphthalmia(MP:0001297)

  • ruptured lens capsule(MP:0008841)

  • small lens(MP:0001306)

  • total cataract(MP:0010263)
    • Detailed phenotype data

    Ordering Information

    Donor DNAChicken delta 1-crystallin (ASL1) enhancer and promoter, Mouse alpha A-crystallin (Cryaa) promoter, Rabbit beta-globin intron, Mouse Sfrp2 coding region, Human growth hormone poly A
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development Biology, 324, 161-176 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYuki Sugiyama (Okinawa Institute of Science and Technology Graduate University (OIST))
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data