Strain Data Sheet

RBRC10239

Strain Information

Image
BRC No.RBRC10239
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Parpbp<tm1.1Chum>
Former Common namePARI(Parpbp) knock-out
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shinichiro Chuma, Institute for Frontier Life and Medical Sciences, Kyoto University in 2017. KY1.1 ES cells derived from (129×C57BL/6)F1 were used. C57BL/6J congenic strain.
Strain descriptionParpbp (PARP1 binding protein) knockout mice. Homozygous mutants are fertile.
Colony maintenance
References
PARI Regulates Stalled Replication Fork Processing To Maintain Genome Stability upon Replication Stress in Mice.
Mochizuki A L, Katanaya A, Hayashi E, Hosokawa M, Moribe E, Motegi A, Ishiai M, Takata M, Kondoh G, Watanabe H, Nakatsuji N, Chuma S
Mol. Cell. Biol., 37(23):e00117-17 (2017). 28894029

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Parpbp
MGI:1922567		PARP1 binding protein10Parpbp<tm1.1Chum> targeted mutation 1, Shinichiro Chuma
loxP phage P1 loxP10loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal DNA replication(MP:0008057)

  • abnormal chromosome stability(MP:0010094)

  • decreased cell proliferation(MP:0000352)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 LoxP site, mouse Parpbp genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Dr. Shinichiro Chuma) using the Approval Form. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a teaching and academic research purpose.
    DepositorShinichiro Chuma (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data