Strain Data Sheet

RBRC10242

Strain Information

Image
BRC No.RBRC10242
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Scn1a<tm2.1Kzy>
Former Common name
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN BSI in 2007. E14 ES cells(129P2/OlaHsd) were used and the Neo gene was deleted by CAG-Flpe mice. C57BL/6J congenic line.
Strain descriptionFloxed mice for the Scn1a (sodium channel, voltage-gated, type I, alpha) gene. A pair of loxP sites flank Exon 7 of the Scn1a.
Colony maintenance
References
Nav1.1 haploinsufficiency in excitatory neurons ameliorates seizure-associated sudden death in a mouse model of Dravet syndrome.
Ogiwara I, Iwasato T, Miyamoto H, Iwata R, Yamagata T, Mazaki E, Yanagawa Y, Tamamaki N, Hensch T K, Itohara S, Yamakawa K
Human Molecular Genetics, 22(23) 4784-4804 (2013). 23922229

Impairments in social novelty recognition and spatial memory in mice with conditional deletion of Scn1a in parvalbumin-expressing cells.
Tetsuya Tatsukawa, Ikuo Ogiwara, Emi Mazaki, Atsushi Shimohata, Kazuhiro Yamakawa
Neurobiol. Dis., 112:24-34 (2018). 29337050

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site2Frt
Scn1a
MGI:98246		sodium channel, voltage-gated, type I, alpha2Scn1a<tm2.1Kzy>
MGI:5523787		targeted mutation 2.1, Kazuhiro Yamakawa
  • developmental and epileptic encephalopathy 6B(MedGEN)

  • Dravet syndrome(DisGeNET, MedGEN)

  • generalized epilepsy with febrile seizures plus, type 2(MedGEN)
    • more 1 Diseases
  • migraine, familial hemiplegic, 3(MedGEN)
    • loxP phage P1 loxP2loxP
    loxP phage P1 loxP2loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, S.cerevisiae FRT(flipase recombination target) site, mouse Scn1a genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Human Molecular Genetics, 22(23), 4784-4804 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKazuhiro Yamakawa (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data