Strain Data Sheet

RBRC10249

update : 2025-02-20

Strain Information
Image
BRC No.	RBRC10249
Type	Targeted Mutation Congenic
Species	Mus musculus
Strain name	B6.Cg-Stx1b<tm1Tfuj>/211
Former Common name	Syntaxin1B
H-2 Haplotype
ES Cell line	TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development	Developed by Tomonori Fujiwara, Kyorin University in 2013. TT2 ES cells were used. Mice were backcrossed to C57BL/6J for 9 generations. This strain was derived from ES clone #211.
Strain description	Stx1b (syntaxin 1B) knockout mice. Homozygous mutants die by postnatal day 14. Heterozygous mutnats are viable and fertile, but prone to develop convulsive seizure.
Colony maintenance
References	HPC-1/syntaxin 1A and syntaxin 1B play distinct roles in neuronal survival. Kofuji T, Fujiwara T, Sanada M, Mishima T, Akagawa K J. Neurochem., 30(4) 514-525 (2014). 24666284 Syntaxin 1B regulates synaptic GABA release and extracellular GABA concentration, and is associated with temperature-dependent seizures. Mishima T, Fujiwara T, Kofuji T, Saito A, Terao Y, Akagawa K J. Neurochem., 156(5) 604-613 (2021). 32858780

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
GH	Growth hormone polyA (Bovine)	7	GH
Stx1b MGI:1930705	syntaxin 1B	7	Stx1b<tm1Tfuj> MGI:5635790	targeted mutation 1, Takefumi Kofuji			generalized epilepsy with febrile seizures plus, type 9(MedGEN)
neo	neomycin resistance gene (E. coli)	7				herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype
Annotation by Mammalian phenotyhpe ontology	abnormal Purkinje cell dendrite morphology(MP:0008572) abnormal hippocampus morphology(MP:0000807) abnormal motor coordination/balance(MP:0001516) cerebellum atrophy(MP:0014185) decreased body size(MP:0001265) more 4 phenotypes embryonic lethality, incomplete penetrance(MP:0011102) impaired righting response(MP:0001523) postnatal lethality, complete penetrance(MP:0011085) thin cerebellar molecular layer(MP:0000890)
Detailed phenotype data

Annotation by Mammalian phenotyhpe ontology

abnormal Purkinje cell dendrite morphology(MP:0008572)

abnormal hippocampus morphology(MP:0000807)

abnormal motor coordination/balance(MP:0001516)

cerebellum atrophy(MP:0014185)

decreased body size(MP:0001265)

more 4 phenotypes

embryonic lethality, incomplete penetrance(MP:0011102)

impaired righting response(MP:0001523)

postnatal lethality, complete penetrance(MP:0011085)

thin cerebellar molecular layer(MP:0000890)

Detailed phenotype data

Ordering Information
Donor DNA	herpes simplex virus thymidine kinase promoter (MC1 promoter), E. coli neo, Bovine growth hormone polyA, mouse HPC-1/syntaxin1A genome DNA
Research application
Specific Term and Conditions	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 30(4) 514-525 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Depositor	Tomonori Fujiwara (Kyorin University)
Strain Status	Frozen sperm
Strain Availability	Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month)
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR-

BRC mice in Publications

No Data