Strain Data Sheet

RBRC10250

Strain Information

Image
BRC No.RBRC10250
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Klre1<tm1Tgi>
Former Common nameNKG2I KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2004. R1 ES cells were used. This line was backcrossed with C57BL/6J for 6 generations.
Strain descriptionKlre1 (killer cell lectin-like receptor family E member 1) knockout mice. Homozygous mutants display impaired natural killer cell mediated allogeneic cytotoxicity. EGFP is placed downstream of translation initiation codon.
Colony maintenance
References
Role of a NK receptor, KLRE-1, in bone marrow allograft rejection: analysis with KLRE-1-deficient mice.
Shimizu E, Koike J, Wakao H, Seino K, Koseki H, Kakiuchi T, Nakayama T, Taniguchi M
Blood, 104, 781-783 (2004). 15069013

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
Klre1
MGI:2662547		killer cell lectin-like receptor family E member 16Klre1<tm1Tgi> targeted mutation 1, Masaru Taniguchi
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene6 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • impaired natural killer cell mediated cytotoxicity(MP:0005070)
    • Detailed phenotype data

    Ordering Information

    Donor DNAAequorea victoria EGFP cDNA, Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Klre1 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood 104, 781-783, (2004).
    DepositorMasaru Taniguchi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data