Strain Data Sheet

RBRC10251

Strain Information

Image
BRC No.RBRC10251
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129-Klre1<tm1Tgi>
Former Common nameNKG2I KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainBALB/cAnNCrlCrlj
Appearance
Strain developmentDeveloped by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2004. R1 ES cells were used. This line was backcrossed with BALB/c for 10 generations.
Strain descriptionKlre1 (killer cell lectin-like receptor family E member 1) knockout mice. Homozygous mutants display impaired natural killer cell mediated allogeneic cytotoxicity. EGFP is placed downstream of translation initiation codon.
Colony maintenance
References
Role of a NK receptor, KLRE-1, in bone marrow allograft rejection: analysis with KLRE-1-deficient mice.
Shimizu E, Koike J, Wakao H, Seino K, Koseki H, Kakiuchi T, Nakayama T, Taniguchi M
Blood, 104, 781-783 (2004). 15069013

Health Report

Examination Date / Room / Rack2024/08/05Room:2-検Rack:FSentinel mouse program
2024/05/07Room:2-検Rack:FSentinel mouse program
2024/02/05Room:2-検Rack:FSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
Klre1
MGI:2662547		killer cell lectin-like receptor family E member 16Klre1<tm1Tgi> targeted mutation 1, Masaru Taniguchi
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • impaired natural killer cell mediated cytotoxicity(MP:0005070)
    • Detailed phenotype data

    Ordering Information

    Donor DNAAequorea victoria EGFP cDNA, Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Klre1 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood 104:781-783, (2004).
    DepositorMasaru Taniguchi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data