Strain Data Sheet

RBRC10252

Strain Information

Image
BRC No.RBRC10252
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Traj18<tm1.1Tgi>
Former Common nameJa18 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2015. B6JN/1 ES cells were used. Traj18 and neo gene fragment were removed by crossing with CAG-cre line. C57BL/6 genetic background.
Strain descriptionTraj18 (T cell receptor alpha joining 18) knockout mice.
Colony maintenance
References
Generation of Novel Traj18-Deficient Mice Lacking Valpha14 Natural Killer T Cells with an Undisturbed T Cell Receptor alpha-Chain Repertoire.
Dashtsoodol N, Shigeura T, Ozawa R, Harada M, Kojo S, Watanabe T, Koseki H, Nakayama M, Ohara O, Taniguchi M
PLoS One 11(4), e0153347 (2016). 27064277

Health Report

Examination Date / Room / Rack2023/08/07Room:2-検Rack:FSentinel mouse program
2023/05/08Room:2-検Rack:FSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Traj18
MGI:4440521		T cell receptor alpha joining 1814Traj18<tm1.1Tgi> targeted mutation 1.1, Masaru Taniguchi
loxP phage P1 loxP14loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased response to antigen(MP:0020001)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Traj18 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One 11(4), e0153347 (2016).
    DepositorMasaru Taniguchi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data