Strain Data Sheet

RBRC10259

Strain Information

Image
BRC No.RBRC10259
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6J. Vα14, Vb7 clone(iPS)
Former Common nameVa14, Vb7 clone
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2014. iPS cells were established by introducing Yamanaka factors into NKT cells derived from B6J spleen. Chimeric mice were generated and then backcrossed with C57BL/6J for 10 generations.
Strain descriptionCloned mice harboring invariant TCR chains, Va14-Ja18 and Vb7.
Colony maintenance
References
Generation of induced pluripotent stem cell-derived mice by reprogramming of a mature NKT cell.
Ren Y, Dashtsoodol N, Watarai H, Koseki H, Quan C, Taniguchi M
Int. Immunol., 26(10), 551-561 (2014). 24854340

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pou5f1
MGI:101893		POU domain, class 5, transcription factor 117Pou5f1
Sox2 SRY (sex determining region Y)-box 23Sox2
Klf4 Kruppel-like transcription factor 4 (gut)4Klf4
Nanog Nanog homeoboxNanog
neo neomycin resistance gene mouse phosphoglycerate kinase promoter (PGK promoter)
Retrovirus LTR
SV40 polyA

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell compartment(MP:0013887)

  • absent gamma-delta T cells(MP:0008348)

  • decreased DN3 thymocyte number(MP:0010134)

  • decreased thymocyte number(MP:0000715)

  • increased CD4-positive NK T cell number(MP:0013520)
    • more 1 phenotypes
  • increased NK T cell number(MP:0008039)
    • Detailed phenotype data

    Ordering Information

    Donor DNARetrovirus LTR, E. coli neomycin, SV40 Poly A, Mouse PGK promoter, Mouse Oct3/4, Mouse Klf-4, Mouse Sox-2, Mouse Nanog
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol. ;26(10), 551-561, (2014)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorMasaru Taniguchi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data