Strain Data Sheet
RBRC10260
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10260 |
| Type | Targeted Mutation and Transgene |
| Species | Mus musculus |
| Strain name | B6J. Vα14 clone(iPS) |
| Former Common name | Va14 clone |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2014. iPS cells were established by introducing Yamanaka factors into NKT cells derived from B6J spleen. Chimeric mice were generated and then backcrossed with C57BL/6J for 10 generations. |
| Strain description | Cloned mice harboring invariant TCR chain, Va14-Ja18. |
| Colony maintenance | |
| References | Generation of induced pluripotent stem cell-derived mice by reprogramming of a mature NKT cell. Ren Y, Dashtsoodol N, Watarai H, Koseki H, Quan C, Taniguchi M Int. Immunol., 26(10), 551-561 (2014). 24854340 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2023/08/07Room:2-検Rack:FSentinel mouse program 2023/05/08Room:2-検Rack:FSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Klf4 | Kruppel-like transcription factor 4 (gut) | UN | Klf4 | ||||
| Nanog | Nanog homeobox | UN | Nanog | ||||
| Pou5f1 MGI:101893 | POU domain, class 5, transcription factor 1 | UN | Pou5f1 | ||||
| Sox2 | SRY (sex determining region Y)-box 2 | UN | Sox2 | ||||
| Retrovirus LTR | UN | ||||||
| SV40 polyA | UN | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | Retrovirus LTR, SV40 Poly A, Mouse PGK promoter, Mouse Oct3/4, Mouse Klf-4, Mouse Sox-2, Mouse Nanog. |
| Research application | Immunology and Inflammation Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol. ;26(10), 551-561, (2014). |
| Depositor | Masaru Taniguchi (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |