Strain Data Sheet
RBRC10262
Strain Information | |
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| Image | |
| BRC No. | RBRC10262 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6J.Cα-IRES Venus KI, Vα14, Vb8.1 clone |
| Former Common name | NKT clone |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2010. ES cells were established by the nuclear transfer of NKT cells into enucleated eggs derived from B6D2F1. IRES-Venus was inserted downstream of the TCR Ca stop codon in the NKT-ES cells. Chimeric mice were generated and then backcrossed with C57BL/6J for 10 generations. |
| Strain description | Cloned mice harboring invariant TCR chains, Va14-Ja18 and Vb8.1. Venus expression from the TCRa locus. |
| Colony maintenance | |
| References | Generation of functional NKT cells in vitro from embryonic stem cells bearing rearranged invariant Valpha14-Jalpha18 TCRalpha gene. Watarai H, Rybouchkin A, Hongo N, Nagata Y, Sakata S, Sekine E, Dashtsoodol N, Tashiro T, Fujii S, Shimizu K, Mori K, Masuda K, Kawamoto H, Koseki H, Taniguchi M Blood, 115(2), 230-237 (2010). 19897575 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| IRES | internal ribosomal entry site (EMCV) | ||||||
| Venus | Yellow Fluorescent Protein (Aequorea victoria) | Venus | |||||
| loxP | phage P1 loxP | loxP | |||||
| neo | neomycin resistance gene | ||||||
| SV40 polyA | |||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Phage P1 loxP, ECMV IRES, Aequorea victoria Venus, SV40 SV40 polyA, SV40 SV40 polyA promoter, E. coli neomycin |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood. 115(2), 230-237, (2010). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Masaru Taniguchi (RIKEN) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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