Strain Data Sheet

RBRC10263

Strain Information

Image
BRC No.RBRC10263
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ppm1b<tm1.1Tamu>
Former Common namePpm1bΔ/wt mouse, PP2CβΔ/wt mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Motoko Ohnishi, Chubu University. Developed by Shinri Tamura, Tohoku University, in collaboration with Jun-ichi Miyazaki, Osaka University. ES cells derived from 129SV were used and then the mice were backcrossed to C57BL/6N over 36 generations. Exon 4 was deleted at ES cells.
Strain descriptionPpm1b (protein phosphatase 1B, magnesium dependent, beta isoform) knockout mice. Exon 4 is deleted. Homozygous mutants are lethal at around embryonic day 3.5.
Colony maintenance
References
Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality.
Sasaki M, Ohnishi M, Tashiro F, Niwa H, Suzuki A, Miyazaki J, Kobayashi T, Tamura S
Mech. Dev., 124(6), 489-499 (2007). 17499977

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ppm1b
MGI:101841		protein phosphatase 1B, magnesium dependent, beta isoform17Ppm1b<tm1.1Tamu>
MGI:3712852		targeted mutation 1.1, Shinri Tamura
lox71 mutant lox sequence17lox71
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal embryonic tissue morphology(MP:0002085)

  • embryonic lethality before implantation, complete penetrance(MP:0011094)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, lox71
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mech Dev., 124(6), 489-499 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorMotoko Ohnishi (Chubu University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data