Strain Data Sheet

RBRC10266

Strain Information

Image
BRC No.RBRC10266
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ppm1l<tm1Tkoba>/79
Former Common namePpm1llox/wt mouse, PP2Cεlox/wt mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Motoko Ohnishi, Chubu University. Developed by Shinri Tamura, Tohoku University, in collaboration with RIKEN CDB in 2006-2007 (CDB0428K). This strain was backcrossed to C57BL/6N over 28 generations.
Strain descriptionPpm1l [protein phosphatase 1 (formerly 2C)-like] knockout mice. The b-galactosidase(LacZ) and neomycin gene cassette were inserted into the 50 UTR sequence of exon 1. Homozygous mutants were born less than the mendelian ratio and died by 4 weeks old. ES clone #79 was used to generate this line.
Colony maintenance
References
Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain.
Kusano R, Fujita K, Shinoda Y, Nagaura Y, Kiyonari H, Abe T, Watanabe T, Matsui Y, Fukaya M, Sakagami H, Sato T, Funahashi J I, Ohnishi M, Tamura S, Kobayashi T
FEBS Lett., 590(20), 3606-3615 (2016). 27680501

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site3Frt
Frt yeast FRT (flippase recombination target) site3Frt
Ppm1l
MGI:2139740		protein phosphatase 1 (formerly 2C)-like3Ppm1l<tm1Tkoba> targeted mutation 1, Takayasu Kobayashi
lacZ beta-galactosidase (E. coli)3
lox71 mutant lox sequence3lox71
loxP phage P1 loxP3loxP
neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal anterior commissure morphology(MP:0008225)

  • abnormal dorsal striatum morphology(MP:0004102)

  • abnormal forebrain morphology(MP:0000783)

  • decreased anterior commissure size(MP:0008226)

  • decreased corpus callosum size(MP:0000781)
    • more 10 phenotypes
  • decreased myelin sheath thickness(MP:0011731)

  • decreased striatum size(MP:0012468)

  • decreased survivor rate(MP:0008770)

  • dysmyelination(MP:0013438)

  • enlarged lateral ventricles(MP:0008535)

  • impaired coordination(MP:0001405)

  • impaired limb coordination(MP:0001524)

  • limb grasping(MP:0001513)

  • postnatal lethality, complete penetrance(MP:0011085)

  • postnatal lethality, incomplete penetrance(MP:0011086)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 lox71 site, E. coli lacZ, rabbit beta-globin 3’end, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neo, phage P1 loxP site, PGK3’end, mouse Ppm1l genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., 590(20), 3606-3615 (2016).
    DepositorMotoko Ohnishi (Chubu University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data