Strain Data Sheet

RBRC10269

Strain Information

Image
BRC No.RBRC10269
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-floxed EGFP,-rtTA,-tTS)1Kyot
Former Common nameB6-cTg/rtTA
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Itoh, Kyoto University in 2015. DNA construct was injected to C57BL/6. C57BL/6 genetic background. Homozygous mice are viable and fertile.
Strain descriptionTransgenic mice in which rtTA expression is dependent on Cre recombinase. Transgenic construct is CAG-loxP-nlsEGFP-loxP-rtTA-IRES-tTS-Kid.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)4EGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
GH Growth hormone polyA (Bovine)4GH
Ires internal ribosomal entry site (EMCV)4
loxP phage P1 loxP4loxP
loxP phage P1 loxP4loxP
nls SV40 large T antigen nuclear localization signal (NLS)4nls
rtTA E. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)4rtTA
tTS E. coli Tetracycline repressor (tetR), rat Kid1 (ZFP KRAB domain) cDNA4tTS
SV40 polyA4

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 loxP sites, SV40 large T antigen nuclear localization signal (NLS), Jellyfish EGFP cDNA, SV40 polyA signal, E. coli TetR mutant (rtTA), Synthetic DNA (Synthetic intron sequence), EMC (encephalomyocarditis virus)internal ribosome entry site, tTS (E. coli Tetracycline repressor (tetR), rat Kid1 (ZFP KRAB domain) cDNA), bovine growth hormone polyA signal
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsThe　availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Itoh (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data