Strain Data Sheet

RBRC10280

Strain Information

Image
BRC No.RBRC10280
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Ahcyl1<tm1Kmik>/1
Former Common nameIRBIT flox mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2009. TT2 ES cells were used and this subline #1 was backcrossed to C57BL/6 for 13 generations. C57BL/6 congenic line.
Strain descriptionhcyl1 (S-adenosylhomocysteine hydrolase-like 1) floxed mice. FRT-flanked Neo gene is placed at intron 1 of the Ahcyl1 gene. A pair of loxP sites flank the FRT-flanked Neo and exon 2 of the Ahcyl1 gene. TT2 ES cells were used and this subline #1 was backcrossed to C57BL/6 for 13 generations. C57BL/6 congenic line.
Colony maintenance
References
IRBIT suppresses IP3 receptor activity by competing with IP3 for the common binding site on the IP3 receptor.
Ando H, Mizutani A, Kiefer H, Tsuzurugi D, Michikawa T, Mikoshiba K
Mol Cell. 22(6):795-806 (2006). 16793548

IRBIT, an inositol 1,4,5-trisphosphate receptor-binding protein, specifically binds to and activates pancreas-type Na+/HCO3- cotransporter 1 (pNBC1).
Shirakabe K, Priori G, Yamada H, Ando H, Horita S, Fujita T, Fujimoto I, Mizutani A, Seki G, Mikoshiba K
Proc. Natl. Acad. Sci. USA, 103(25):9542-9547 (2006). 16769890

IRBIT regulates CaMKIIalpha activity and contributes to catecholamine homeostasis through tyrosine hydroxylase phosphorylation.
Kawaai K, Mizutani A, Shoji H, Ogawa N, Ebisui E, Kuroda Y, Wakana S, Miyakawa T, Hisatsune C, Mikoshiba K
Proc. Natl. Acad. Sci. USA, 112(17):5515-5520 (2015). 25922519

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ahcyl1
MGI:2385184		S-adenosylhomocysteine hydrolase-like 13Ahcyl1<tm1Kmik> targeted mutation 1, Katsuhiko Mikoshiba
Frt yeast FRT (flippase recombination target) site3Frt
Frt yeast FRT (flippase recombination target) site3Frt
loxP phage P1 loxP3loxP
loxP phage P1 loxP3loxP
neo neomycin resistance gene3 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal social recognition(MP:0020396)

  • abnormal social/conspecific interaction behavior(MP:0002557)

  • decreased body weight(MP:0001262)

  • hyperactivity(MP:0001399)

  • increased brain tyrosine 3-monooxygenase activity(MP:0020076)
    • more 3 phenotypes
  • increased dopamine level(MP:0001906)

  • increased noradrenaline level(MP:0012575)

  • proportional dwarf(MP:0008974)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, Saccharomyces cerevisiae frt sites, mouse PGK promoter, Escherichia coli neomycin-resistant gene (Pro/Neo/3'end), mouse PGK pA, mouse Ahcyl1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Mikoshiba (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data