Strain Data Sheet

RBRC10290

Strain Information

Image
BRC No.RBRC10290
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Itpr3<tm1Kmik>
Former Common nameIP3R3 knockout mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2005. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1 were used. C57BL/6J congenic line by crossing with C57BL/6 for 10 generations.
Strain descriptionItpr3 (inositol 1,4,5-trisphosphate receptor 3) KO mouse. A nls-LacZ gene fragment was inserted downstream of the translation initiation codon. Homozygous mutant exhibit abnormal hair loss and low sweet preference.
Colony maintenance
References
IP3 receptor types 2 and 3 mediate exocrine secretion underlying energy metabolism.
Futatsugi A, Nakamura T, Yamada M K, Ebisui E, Nakamura K, Uchida K, Kitaguchi T, Takahashi-Iwanaga H, Noda T, Aruga J, Mikoshiba K
Science, 309(5744) 2232-2234 (2005). 16195467

Decreased olfactory mucus secretion and nasal abnormality in mice lacking type 2 and type 3 IP3 receptors.
Fukuda N, Shirasu M, Sato K, Ebisui E, Touhara K, Mikoshiba K
Eur. J. Neurosci., 27(10) 2665-2675 (2008). 18547250

Regulation of hair shedding by the type 3 IP3 receptor.
Sato-Miyaoka M, Hisatsune C, Ebisui E, Ogawa N, Takahashi-Iwanaga H, Mikoshiba K
J. Invest. Dermatol., 132(9) 2137-2147 (2012). 22572820

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:DSentinel mouse program
2024/10/21Room:3-CRack:DSentinel mouse program
2024/04/22Room:3-CRack:DSentinel mouse program
2024/01/22Room:3-CRack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Itpr3
MGI:96624		inositol 1,4,5-triphosphate receptor 317Itpr3<tm1Kmik>
MGI:3604625		targeted mutation 1, Katsuhiko Mikoshiba
  • type 1 diabetes mellitus(MedGEN)
    • neo neomycin resistance gene17 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal hair cycle catagen phase(MP:0008859)

  • abnormal hair cycle(MP:0000427)

  • abnormal hair follicle bulge morphology(MP:0009759)

  • abnormal hair follicle development(MP:0003704)

  • abnormal keratinocyte adhesion(MP:0030576)
    • more 4 phenotypes
  • absent active-zone-anchored inner hair cell synaptic ribbon(MP:0004563)

  • alopecia(MP:0000414)

  • disheveled coat(MP:0001511)

  • focal dorsal hair loss(MP:0010202)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse PGK promoter, Escherichia coli neomycin-resistant gene, mouse PGK pA, Mouse Itpr3 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Mikoshiba (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data