Strain Data Sheet

RBRC10291

Strain Information

Image
BRC No.RBRC10291
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Itpr2<tm1Kmik> Itpr3<tm1Kmik>
Former Common nameIP3R2 IP3R3 double knockout mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2005. J1 ES cells derived from 129S4/SvJae were used for Itpr2. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1 were used for Itpr3. C57BL/6J congenic line by crossing with C57BL/6 for 10 generations.
Strain descriptionDouble KO line for Itpr2 (inositol 1,4,5-trisphosphate receptor 2) and Itpr3 (inositol 1,4,5-triphosphate receptor 3). Each modified allele is derived from RBRC10289 and RBRC10290.
Colony maintenance
References
IP3 receptor types 2 and 3 mediate exocrine secretion underlying energy metabolism.
Futatsugi A, Nakamura T, Yamada M K, Ebisui E, Nakamura K, Uchida K, Kitaguchi T, Takahashi-Iwanaga H, Noda T, Aruga J, Mikoshiba K
Science, 309(5744) 2232-2234 (2005). 16195467

Decreased olfactory mucus secretion and nasal abnormality in mice lacking type 2 and type 3 IP3 receptors.
Fukuda N, Shirasu M, Sato K, Ebisui E, Touhara K, Mikoshiba K
Eur. J. Neurosci., 27(10) 2665-2675 (2008). 18547250

Abolished InsP3R2 function inhibits sweat secretion in both humans and mice.
Klar J, Hisatsune C, Baig S M, Tariq M, Johansson A C, Rasool M, Malik N A, Ameur A, Sugiura K, Feuk L, Mikoshiba K, Dahl N
J. Clin. Invest., 124(11) 4773-4780 (2014). 25329695

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:LSentinel mouse program
2024/10/21Room:3-CRack:LSentinel mouse program
2024/04/22Room:3-CRack:LSentinel mouse program
2024/01/22Room:3-CRack:LSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Itpr3
MGI:96624		inositol 1,4,5-triphosphate receptor 317Itpr3<tm1Kmik>
MGI:3604625		targeted mutation 1, Katsuhiko Mikoshiba
  • type 1 diabetes mellitus(MedGEN)
    • neo neomycin resistance gene17 mouse phosphoglycerate kinase promoter (PGK promoter)
    Itpr2
    MGI:99418    		inositol 1,4,5-triphosphate receptor 26Itpr2<tm1Kmik>
    MGI:3604623    		targeted mutation 1, Katsuhiko Mikoshiba
  • isolated anhidrosis with normal sweat glands(MedGEN)
    • lacZ beta-galactosidase (E. coli)6
    neo neomycin resistance gene6 mouse phosphoglycerate kinase promoter (PGK promoter)
    nls SV40 large T antigen nuclear localization signal (NLS)6nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal feces composition(MP:0003868)

  • abnormal food intake(MP:0005449)

  • abnormal pancreas secretion(MP:0002694)

  • abnormal pancreatic acinar cell morphology(MP:0009146)

  • abnormal salivary gland physiology(MP:0005310)
    • more 11 phenotypes
  • abnormal sweat gland physiology(MP:0013368)

  • decreased body weight(MP:0001262)

  • decreased circulating glucose level(MP:0005560)

  • decreased salivation(MP:0000623)

  • hypohidrosis(MP:0013371)

  • increased defecation amount(MP:0003867)

  • pancreatic acinar cell zymogen granule accumulation(MP:0009161)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • steatorrhea(MP:0002791)

  • weight loss(MP:0001263)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse PGK promoter, Escherichia coli neomycin-resistant gene, Mouse PGK pA, SV40 large tumor nuclear location signal, Escherichia coli lacZ gene, Mouse Itpr2 genomic DNA, Mouse Itpr3 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Mikoshiba (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data